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The expression of synapse-associated proteins and glutamate receptor subunit proteins in the CA1 area of 8-month-old WT and APP/PS1 mice. (A) Representative western blots of glutamate receptor subunits. (B–D) Quantitative of NMDAR subunits expression showed a significant decrease of NR2A and NR2B expression ( n = 6 mice/group, * p < 0.05, ** p < 0.01, unpaired t -test) in the hippocampus in 8-month-old APP/PS1 mice compared with WT mice, but there was no difference on the expression of NR1 from two groups. (E,F) Quantitative of AMPA receptors subunits (GluR1, GluR2) expression showed no change in hippocampus from 8-month-old APP/PS1 mice and WT mice. (G) Representative western blots of <t>PSD95</t> and SNAP25. (H,I) A significant decrease of the levels of PSD95 and SNAP25 in the hippocampus from 8-month-old APP/PS1 mice compared with WT mice ( n = 4 mice/group, * p < 0.05, unpaired t -test).
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The expression of synapse-associated proteins and glutamate receptor subunit proteins in the CA1 area of 8-month-old WT and APP/PS1 mice. (A) Representative western blots of glutamate receptor subunits. (B–D) Quantitative of NMDAR subunits expression showed a significant decrease of NR2A and NR2B expression ( n = 6 mice/group, * p < 0.05, ** p < 0.01, unpaired t -test) in the hippocampus in 8-month-old APP/PS1 mice compared with WT mice, but there was no difference on the expression of NR1 from two groups. (E,F) Quantitative of AMPA receptors subunits (GluR1, GluR2) expression showed no change in hippocampus from 8-month-old APP/PS1 mice and WT mice. (G) Representative western blots of <t>PSD95</t> and SNAP25. (H,I) A significant decrease of the levels of PSD95 and SNAP25 in the hippocampus from 8-month-old APP/PS1 mice compared with WT mice ( n = 4 mice/group, * p < 0.05, unpaired t -test).
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The expression of synapse-associated proteins and glutamate receptor subunit proteins in the CA1 area of 8-month-old WT and APP/PS1 mice. (A) Representative western blots of glutamate receptor subunits. (B–D) Quantitative of NMDAR subunits expression showed a significant decrease of NR2A and NR2B expression ( n = 6 mice/group, * p < 0.05, ** p < 0.01, unpaired t -test) in the hippocampus in 8-month-old APP/PS1 mice compared with WT mice, but there was no difference on the expression of NR1 from two groups. (E,F) Quantitative of AMPA receptors subunits (GluR1, GluR2) expression showed no change in hippocampus from 8-month-old APP/PS1 mice and WT mice. (G) Representative western blots of <t>PSD95</t> and SNAP25. (H,I) A significant decrease of the levels of PSD95 and SNAP25 in the hippocampus from 8-month-old APP/PS1 mice compared with WT mice ( n = 4 mice/group, * p < 0.05, unpaired t -test).
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The expression of synapse-associated proteins and glutamate receptor subunit proteins in the CA1 area of 8-month-old WT and APP/PS1 mice. (A) Representative western blots of glutamate receptor subunits. (B–D) Quantitative of NMDAR subunits expression showed a significant decrease of NR2A and NR2B expression ( n = 6 mice/group, * p < 0.05, ** p < 0.01, unpaired t -test) in the hippocampus in 8-month-old APP/PS1 mice compared with WT mice, but there was no difference on the expression of NR1 from two groups. (E,F) Quantitative of AMPA receptors subunits (GluR1, GluR2) expression showed no change in hippocampus from 8-month-old APP/PS1 mice and WT mice. (G) Representative western blots of <t>PSD95</t> and SNAP25. (H,I) A significant decrease of the levels of PSD95 and SNAP25 in the hippocampus from 8-month-old APP/PS1 mice compared with WT mice ( n = 4 mice/group, * p < 0.05, unpaired t -test).
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The expression of synapse-associated proteins and glutamate receptor subunit proteins in the CA1 area of 8-month-old WT and APP/PS1 mice. (A) Representative western blots of glutamate receptor subunits. (B–D) Quantitative of NMDAR subunits expression showed a significant decrease of NR2A and NR2B expression ( n = 6 mice/group, * p < 0.05, ** p < 0.01, unpaired t -test) in the hippocampus in 8-month-old APP/PS1 mice compared with WT mice, but there was no difference on the expression of NR1 from two groups. (E,F) Quantitative of AMPA receptors subunits (GluR1, GluR2) expression showed no change in hippocampus from 8-month-old APP/PS1 mice and WT mice. (G) Representative western blots of <t>PSD95</t> and SNAP25. (H,I) A significant decrease of the levels of PSD95 and SNAP25 in the hippocampus from 8-month-old APP/PS1 mice compared with WT mice ( n = 4 mice/group, * p < 0.05, unpaired t -test).
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The expression of synapse-associated proteins and glutamate receptor subunit proteins in the CA1 area of 8-month-old WT and APP/PS1 mice. (A) Representative western blots of glutamate receptor subunits. (B–D) Quantitative of NMDAR subunits expression showed a significant decrease of NR2A and NR2B expression ( n = 6 mice/group, * p < 0.05, ** p < 0.01, unpaired t -test) in the hippocampus in 8-month-old APP/PS1 mice compared with WT mice, but there was no difference on the expression of NR1 from two groups. (E,F) Quantitative of AMPA receptors subunits (GluR1, GluR2) expression showed no change in hippocampus from 8-month-old APP/PS1 mice and WT mice. (G) Representative western blots of <t>PSD95</t> and SNAP25. (H,I) A significant decrease of the levels of PSD95 and SNAP25 in the hippocampus from 8-month-old APP/PS1 mice compared with WT mice ( n = 4 mice/group, * p < 0.05, unpaired t -test).
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The expression of synapse-associated proteins and glutamate receptor subunit proteins in the CA1 area of 8-month-old WT and APP/PS1 mice. (A) Representative western blots of glutamate receptor subunits. (B–D) Quantitative of NMDAR subunits expression showed a significant decrease of NR2A and NR2B expression ( n = 6 mice/group, * p < 0.05, ** p < 0.01, unpaired t -test) in the hippocampus in 8-month-old APP/PS1 mice compared with WT mice, but there was no difference on the expression of NR1 from two groups. (E,F) Quantitative of AMPA receptors subunits (GluR1, GluR2) expression showed no change in hippocampus from 8-month-old APP/PS1 mice and WT mice. (G) Representative western blots of <t>PSD95</t> and SNAP25. (H,I) A significant decrease of the levels of PSD95 and SNAP25 in the hippocampus from 8-month-old APP/PS1 mice compared with WT mice ( n = 4 mice/group, * p < 0.05, unpaired t -test).
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Image Search Results


The expression of synapse-associated proteins and glutamate receptor subunit proteins in the CA1 area of 8-month-old WT and APP/PS1 mice. (A) Representative western blots of glutamate receptor subunits. (B–D) Quantitative of NMDAR subunits expression showed a significant decrease of NR2A and NR2B expression ( n = 6 mice/group, * p < 0.05, ** p < 0.01, unpaired t -test) in the hippocampus in 8-month-old APP/PS1 mice compared with WT mice, but there was no difference on the expression of NR1 from two groups. (E,F) Quantitative of AMPA receptors subunits (GluR1, GluR2) expression showed no change in hippocampus from 8-month-old APP/PS1 mice and WT mice. (G) Representative western blots of PSD95 and SNAP25. (H,I) A significant decrease of the levels of PSD95 and SNAP25 in the hippocampus from 8-month-old APP/PS1 mice compared with WT mice ( n = 4 mice/group, * p < 0.05, unpaired t -test).

Journal: Frontiers in Aging Neuroscience

Article Title: Deficits in N-Methyl-D-Aspartate Receptor Function and Synaptic Plasticity in Hippocampal CA1 in APP/PS1 Mouse Model of Alzheimer’s Disease

doi: 10.3389/fnagi.2021.772980

Figure Lengend Snippet: The expression of synapse-associated proteins and glutamate receptor subunit proteins in the CA1 area of 8-month-old WT and APP/PS1 mice. (A) Representative western blots of glutamate receptor subunits. (B–D) Quantitative of NMDAR subunits expression showed a significant decrease of NR2A and NR2B expression ( n = 6 mice/group, * p < 0.05, ** p < 0.01, unpaired t -test) in the hippocampus in 8-month-old APP/PS1 mice compared with WT mice, but there was no difference on the expression of NR1 from two groups. (E,F) Quantitative of AMPA receptors subunits (GluR1, GluR2) expression showed no change in hippocampus from 8-month-old APP/PS1 mice and WT mice. (G) Representative western blots of PSD95 and SNAP25. (H,I) A significant decrease of the levels of PSD95 and SNAP25 in the hippocampus from 8-month-old APP/PS1 mice compared with WT mice ( n = 4 mice/group, * p < 0.05, unpaired t -test).

Article Snippet: The membrane was blocked in 5% milk-TBST at RT and probed with rabbit anti-NR2A (ab133265, Abcam, United Kingdom; 1:1,000), rabbit anti-NR2B (ab183942, Abcam, United Kingdom; 1:1,000), rabbit anti-NR1 (ab109182, Abcam, United Kingdom; 1:1,000), rabbit anti-GluR1 (04-855, Millipore, Germany; 1:1,000), mouse anti-GluR2 (MAB397, Millipore, Germany; 1:1,000), PSD95 antibody (sc2290, Santa Cruz Biotechnology Inc., United States; 1:500), SNAP25 antibody (A2234, Abclonal, China; 1:500) at 4°C overnight and then reacted with the Alexa Fluor 800 conjugated antibody (1:5,000) for 60 min.

Techniques: Expressing, Western Blot